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Prozyme公司於1990年成立於美國加州,專業開發高質量的試劑、試劑盒及平台,為廣大客戶提供診斷和科研所需的標記物、蛋白質和酶等。Prozyme20031月收購了Glyko®產品線,包括研究糖類的酶及相關試劑。其中包括Glyko®1999年收購的OxfordGlycoSciencesLtd.公司的bob亚洲体育 產品線。

目前,Prozyme公司可提供商品化的GlykoPrep™樣品快速製備平台,該平台大大加速和簡化了糖分析樣品的製備流程,其製備的高品質產品可用於生化製藥藥物開發和生產。
       1. GlykoPrep™糖樣品快速製備平台
        治 療性蛋白質的糖基化譜對其藥理學性質發揮著重要作用,會影響免疫原性、藥代動力學和藥效學。然而,由於目前的手動樣品前處理方法複雜、持續多日,隻能對開 發流程中代表關鍵決策點的樣品進行分析。為了滿足針對生物仿製藥和新型生物藥物開發日益嚴格的法規要求,提供更高的數據質量和分析通量速度,ProZyme推出GlykoPrep™ 樣品快速製備平台,GlykoPrep-plus先進化學試劑能夠實現快速的N-糖鏈消化、標記和淨化,為您提供穩定和可重現的定量結果。
     
        該平台主要以AssayMAP™小柱 為基礎,該小柱包含5μl的填充床,可填充粒徑在20100μm之間的幾乎所有聚合物、矽膠和凝膠型樹脂。樣品杯最大樣品承載體積為200μl。 
        AssayMAP™小柱可放置在特殊的模塊支架上,結合相關的接收板和收集板(96孔式),完成樣品的製備收集。實驗時,樣品、緩衝液及其它試劑加入到樣品杯中,通過微孔板離心機的低速離心處理,溶液流經樹脂填充床。兩塊板同時使用,可最多製備192個樣品。

 

平台特點

·        從純化到聚糖釋放,完成整個樣本製備流程just 3h, not 3-4 days

·        非選擇性,定量釋放及回收完整的N-聚糖

·        選擇靈活,可實現高通量(處理1-192個樣本)

·        室溫標記,無唾液酸損失,標記效率>80%

·        與多種自動化平台的微孔板液體處理係統兼容


 

應用

·        細胞培養優化

·        菌株篩選

·        產品表征

·        質量源於設計中的工藝過程分析

·        質量控製

樣本類型

·        單克隆抗體

·        Fc融合蛋白

·        其他重組糖蛋白

檢測方法

·        HPLC

·        LC/MS

·        CE

·        MS

·        HPAEC-PAD

自動化選擇

·        手動處理

·        液體處理係統,實現半自動化

·        全自動化

平台組成:

·         GlykoPrep™Digestion Module

·         GlykoPrep™Labeling Module

·         GlykoPrep™Cleanup Module

實驗流程示意圖:


圖:GlykoPrepRapid N-Glycan Preparation withInstantAB™實驗流程

相關產品鏈接:

GPPNG-ABGlykoPrep®-plus Rapid N-Glycan Sample Preparation with 2-ABNEW!

GP96NG-AB GlykoPrep™ Rapid N-Glycan Preparation with2-AB

GP96-ABCU GlykoPrep™ Rapid 2-AB Labeling andCleanup

GP96NG-LA GlykoPrep™ Rapid N-Glycan Preparationwith InstantAA

GP96NG-LB GlykoPrep™ Rapid N-Glycan Preparationwith InstantAB

GP96NG-LF GlykoPrep™ Rapid N-GlycanPreparation

GP24NG-AB GlykoPrep™ Rapid N-Glycan Preparationwith 2-AB (24-ct)

GP24-ABCU GlykoPrep™Rapid-Reductive-Amination™ 2-AB Labeling and Cleanup(24-ct)

GP24NG-LA GlykoPrep™ Rapid N-Glycan Preparationwith InstantAA™ (24-ct)

GP24NG-LB GlykoPrep™ Rapid N-Glycan Preparationwith InstantAB

GP24NG-LF GlykoPrep™ Rapid N-Glycan Preparation(24-ct)

Gilson®TOOLKIT

Equipment andSupplies

2.Glyko®糖生物學研究工具

糖苷酶

1.內切糖苷酶

Code

Description

Size/Qty

N-Glycanase

GKE-5006B

N-Glycanase® (recombinant gene fromElizabethkingia meningoseptica, expressed in E.coli)

1 ea (200mU in 80µl)

O-Glycanase

GK80090

Glyko® O-Glycanase (recombinant genefrom Streptococcus pneumoniae, expressed inE.coli)

1 ea (50 mU (40µl))


2. 外切糖苷酶

Code

Description

Size/Qty

Sialidase

GK80043

Glyko® Sialidase A™ SamplerKit

1 ea (1ea)

GK80040

Glyko® Sialidase A(recombinant gene fromArthrobacter ureafaciens, expressed in E.coli)

1 ea (1 U (200µl))

BetaGalactosidase

GK80120

Glyko® β(1,3,6)-Galactosidase/GALaseII (recomb from Xanthomonas manihotis, expressed in E.coli)

1 ea (200 mU (40µl))

Hexosaminidase

GKX-5003

Glyko® β-N-Acetylhexosaminidase (jackbean)/HEXase III

1 ea (5 U)

Galactosaminidase

GKX-5001

Glyko® α-N-Acetylgalactosaminidase(chicken liver)

1 ea (100mU)

AlphaMannosidase

GKX-5010

Glyko® α(1-2,3,6)-Mannosidase (jackbean)

1 ea (10U)

BetaMannosidase

GKX-5015

Glyko® β-Mannosidase (Helixpomatia)

1 ea (1 Ulyophilized)

AlphaFucosidase

GK80170

Glyko® α(1-2)Fucosidase/FUCase II(Xanthomonas manihotis)

1 ea (4mU)

 

·         糖蛋白去糖基化試劑盒

Code

Description

Size/Qty

EnzymaticDeglycosylation

GK80110

GlycoProTM Enzymatic DeglycosylationKit for N-Linked & Simple O-Linked Glycans

1 ea (1 kit (~2 mg ofglycoprotein))

ChemicalDeglycosylation

GKK-500

GlycoFreeTM Chemical DeglycosylationKit

1 ea (1 kit up to 24samples (2 sets of 12))

 

·         GlykoScreenTM唾液酸定量檢測

Code

Description

Size/Qty

GS300

GlykoScreenTM Rapid Sialic AcidQuantitation Kit

1 ea (1kit)

GF57

Sialic Acid QuantitationKit

1 ea (kit)

GS300LA

GlykoScreenTM Sialic AcidQuantitation Kit

1 ea (1kit)

GS300WD

GlykoScreenTM Sialic AcidQuantitation Kit

1 ea (1kit)

 

·         SignalTM標記試劑盒

Code

Description

Size/Qty

GKK-402

Glyko® Signal™ 2-AA LabelingKit

1 ea (2 sets (2 x 18rxns))

GKK-404

Glyko® Signal™ 2-AB LabelingKit

1 ea (2 sets (2 x 18rxns))

GKK-407

Glyko® Signal™ DMB Sialic Acid LabelingKit

1 ea (2 sets (2 x 10rxns))

 

·         糖類洗滌

Code

Description

Size/Qty

GlykoCleanClean-upStation

GC100

GlykoClean™ Glycan Clean-upStation

1 ea (ea)

GlykoCleanRapid Clean-UpCartridges

GC210

GlykoClean™ S-plusCartridges

1 ea (25-countpack)
4 ea (25-countpack)

GC250

GlykoClean™ GCartridges

1 ea (25-countpack)
4 ea (25-countpack)

GlycoCleanCarbohydrate Clean-UpCartridges

GKI-4025

Glyko® GlycoClean™ HCartridges

1 ea (10cartridges)

GKI-4756

Glyko® GlycoCleanRCartridges

1 ea (12cartridges)

GKI-4726

Glyko® GlycoClean™ SCartridges

1 ea (12cartridges)

 

·         糖標準品

Code

Description

Size/Qty

N-LinkedOligosaccharides

GKC-004301

Glyko™ Asialo-, agalacto-,biantennary, core-substituted with fucose(NGA2F)

1 ea (10 µg)
2 ea (20 µg (2x 10 µg) )

GKC-005300

Glyko™ Asialo-, agalacto-,triantennary (NGA3)

1 ea (10µg)
2 ea (20µg)

GKC-006300

Glyko™ Asialo-, agalacto-,tetraantennary (NGA4)

1 ea (20µg)

GKM-002500

Glyko® Oligomannose 5(Man-5)

1 ea (10µg)
2 ea (20 µg (2x 10 µg)µ)

GKC-224300

Glyko™ Di-sialylated-,galactosylated biantennary (A2)

1 ea (10 µg (1 x 10µg))
2 ea (10 µg)

InstantABLabeledOligosaccharides

GKIB-503

Glyko® InstantAB™-(GlucoseHomopolymer)

1 ea (200pmol)

GKIB-005

Glyko® InstantAB™ Human IgG N-Linked GlycanLibrary

1 ea(70pmol)

GKIB-009

Glyko® InstantAB™-(RNAse B N-Linked GlycanLibrary)

1 ea(70pmol)

GKIB-002

Glyko® InstantAB™-(Bovine Fetuin N-LinkedGlycan Library)

1 ea(70pmol)

GKIB-001

Glyko® InstantAB™-(Human α1-Acid Glycoprotein N-LinkedGlycan Library)

1 ea (70pmol)

InstantAALabeledOligosaccharides

GKIA-503

Glyko® InstantAA™ Glucose HomopolymerStandard

1 ea (200pmol)

Hybrid-typeOligosaccharides

GKH-003510

Glyko® Hybrid-type N-Glycan withbisecting GlcNAc

1 ea (10µg)

N-Linked Cores

GKR-002000

Glyko® Chitobiose(NN)

1 ea (20µg)

GKR-002100

Glyko® 4'-β-mannosylchitobiose (MNN,MAN-1)

1 ea (10µg)

GKR-002300

Glyko® Conserved trimannosyl core(M3N2, MAN-3)

1 ea (10µg)
2 ea (20 µg (2x 10 µg))

Neutral O-LinkedOligosaccharides

GKAD-00125

Fucosyl-p-lacto-N-hexaose

1 ea (100µg)

GKAD-00131

Glyko® Difucosyllacto-N-hexaose(DFLNH(a))

1 ea (100µg)

GKAD-00133

Glyko® Monofucosyllacto-N-hexaoseIII (MFLNH-III)

1 ea (100µg)

GKAD-00135

Glyko® Trifucosyllacto-N-hexaose(TFLNH)

1 ea (100µg)

Sialylated O-LinkedOligosaccharides

GKAD-00116

Glyko® 3'-Sialyl-3-fucosyllactose(3'-S,3-FL)

1 ea (100µg)

GKAD-01014

Glyko® 3'-Sialyl-N-acetyllactosamine(3'-SLN)

1 ea (500µg)

GKAD-01015

Glyko® 6'-Sialyl-N-acetyllactosamine(6'-SLN)

1 ea (500µg)

BloodGroup Oligosaccharides

   

GKAD-00141

Glyko® Blood group Atrisaccharide

1 ea (5mg)

   

GKAD-00143

Glyko® Blood group Btrisaccharide

1 ea (5mg)

   

GKAD-00144

Glyko® Blood group Hdisaccharide

1 ea (1mg)

   

LewisAntigens

   

GKAD-00126

3'-Sialyl Lewis x

1 ea (100µg)

   

GKAD-00127

Lewis x

1 ea (100µg)

   

GKAD-00128

3'-Sialyl Lewis a

1 ea (100µg)

   

GKAD-00129

Lewis a

1 ea (100µg)

   

Sialylated Oligosaccharides found inHuman Milk

   

GKGS-312

GlycoSet II

1 ea (ea )

   

2-AB(2-Aminobenzamide) Labeled Oligosaccharides

   

GKSB-103

Glyko® 2-AB oligomannose 5 (2-ABMan-5)

1 ea (100pmol)
6 ea (100pmol)

 

GKSB-301

Glyko® 2-AB asialo-, agalacto-,biantennary (2-AB NGA2)

1 ea (100pmol)
6 ea (100pmol)

   

GKSB-304

Glyko® 2-AB asialo-, galactosylatedbiantennary (2-AB NA2)

1 ea (100pmol)
6 ea (100pmol)

   

GKSB-307

Glyko® 2-AB asialo-, agalacto-,triantennary (2-AB NGA3)

1 ea (100pmol)
6 ea (100pmol)

   

2-AA(2-Aminobenzoic acid) Labeled Oligosaccharides

   

GKSA-103

Glyko® 2-AA Oligomannose 5 (2-AAMan-5)

1 ea (100pmol)
6 ea (100pmol)

   

GKSA-304

Glyko® 2-AA asialo, galactosylatedbiantennary (2-AA NA2)

1 ea (100pmol)
6 ea (100pmol)

   
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